What do you need to pay attention to in ELISA experiments?

Key Points to Consider

Please refer to the detailed instructions.

Follow these steps carefully:

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1. Washing Solution:

It is recommended to prepare the washing solution before use. Some kits specify that it can be stored at 4°C for up to one month. If the kit instructions do not mention storage time, it's better to prepare a fresh solution each time. Avoid over-preparing unless necessary.

2. Staining Solution:

The staining solution is typically divided into two parts: Liquid A and Liquid B. It’s best to prepare them within the first 15 minutes of use to ensure optimal performance.

3. Standards:

Most ELISA kits come with pre-prepared standards. Store them at 4°C. However, some kits may require you to prepare the standards yourself, so always check the kit instructions for specific guidance.

4. Concentrated Biotin-Conjugated Secondary Antibody and HRP:

If the kit instructions don’t specify how long the reagent can be stored after preparation, it’s best to use it immediately. Some products come in small tubes that should be centrifuged before opening to prevent contamination or excess volume when the lid is removed.

5. Loading Reagents:

When incubating reagents at room temperature, especially those with short incubation times, the way you load the reagents can significantly affect your results. Be careful and consistent during this step.

ELISA experiments are widely used in scientific research due to their high sensitivity and specificity. They have become an essential tool in many research projects. However, if any step is not performed carefully, it can greatly impact the final outcome. Neglecting details such as improper washing, incorrect staining, or poor loading can lead to issues like weak color development or uneven plate readings.

Shanghai Jiwei Biotechnology Co., Ltd. has been specializing in ELISA kits for many years. With advanced technology and equipment, we provide reliable reagents to support scientific research across China. If you encounter any difficulties while performing ELISA experiments, feel free to contact us anytime. Our technical team will be happy to assist you and answer all your questions in detail.