What do you need to pay attention to in ELISA experiments?

EL-C1600N100013-B

Spot Remarks 2017+

The probe capacitor polarity needle has a head diameter of 5.8. The total length of the 7-pin and 12-pin needles is 5.

Brand AVX TPSE226M035R0125 Low impedance tantalum capacitor AVX 22

Key Points to Note

Please refer to the details for more information.

Follow these steps carefully:

1. Washing Solution:

Prepare it before use. Some kits specify that the washing solution should be prepared fresh, while others may allow storage at 4°C for up to one month. If the instructions don't mention this, it's better to prepare it freshly. Avoid over-preparing unless necessary.

2. Staining Solution:

This usually consists of two parts: Liquid A and Liquid B. It’s best to prepare them within the first 15 minutes of use for optimal results.

3. Standards:

Most ELISA kits come with pre-prepared standards. Store them at 4°C. However, some may require immediate preparation according to the kit instructions.

4. Concentrated Biotin-Conjugated Secondary Antibody and HRP:

If required, follow the kit instructions. If no storage duration is specified, use the reagent immediately. Some preparations need to be used within 15 minutes. Always check the instruction manual for specific guidance. Before opening, centrifuge the small tubes to avoid contamination and maintain accuracy.

5. Loading Reagents:

For reagents that require incubation at room temperature, especially those with short incubation times, proper loading is crucial. Improper loading can significantly affect your experimental results.

ELISA experiments are widely used in scientific research due to their high sensitivity and specificity. They have become an essential tool in many research projects. However, even small mistakes during any step can greatly impact the final outcome. Neglecting details may lead to issues such as weak color development or uneven plate readings.

Shanghai Jiwei Biotechnology Co., Ltd. has been specializing in ELISA kits for many years. With advanced technology and state-of-the-art equipment, we provide reliable and high-quality reagents for scientific research in China. If you encounter any difficulties during your ELISA experiments, feel free to contact us anytime. Our technical team will be happy to assist you with detailed guidance and support.